Determination of microbial genome sizes by two-dimensional denaturing gradient gel electrophoresis.

Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis

PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...

Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

Denaturing Gradient Gel Electrophoresis – a Tool for Plant Protection Research

Analysis of microbial communities associated with plants, insects and soil has been a significant challenge for plant protection researchers, because of the lack of techniques with which to access these populations. A new molecular community profiling technique, 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), has been used to analyse microbial communities in ...

Microbial Community Fingerprints by Differential Display Denaturing Gradient 1 Gel Electrophoresis ( DD - DGGE )

Seasonal variations of microbial community in a full scale oil field produced water treatment plant

This study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. The community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and Illumina high-throughput sequencing, respectively. Chemical oxygen demand eff...